General Cell Based Assays
Cell Biolabs Inc CytoSelect™ 24-Well Cell Invasion Assay Kit (Collagen I, Colorimetric), 12 assays
Cell invasion is the ability of malignant tumor cells to invade normal surrounding tissue, which contributes in large part to the significant morbidity and mortality of cancers. The CytoSelect™ Cell Invasion Assay Kit utilizes polycarbonate membrane inserts (8 µm pore size) to assay the invasive properties of cells. The kit contains twelve 8 um polycarbonate membrane inserts coated with a Collagen gel layer in a 24-well plate. Cells must degrade the Collagen layer and then move through the pores of the membrane. Invasive cells are then quantified using the provided Cell Stain Solution.
Bioassay Systems EnzyFluo™ Bile Acid Assay Kit. For quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism.
For quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. Linear detection range of 1 - 150 µM bile acids. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: Serum, plasma, urine and other biological samples. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 1 µM.
Eurofins Discoverx PathHunter eXpress BAI2 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0546E2CP1L)
The PathHunter BAI2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of BAI2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BAI2 and EA-tagged β-Arrestin-2. Activation of BAI2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress DARC CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0548E2CP1L)
The PathHunter DARC eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of DARC GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DARC and EA-tagged β-Arrestin-2. Activation of DARC stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress BAI3 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0547E2ACP1M)
The PathHunter BAI3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of BAI3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BAI3 and EA-tagged β-Arrestin-2. Activation of BAI3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HRH3 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0509E2CP2M)
The PathHunter HRH3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. histamine) based activation of HRH3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HRH3 and EA-tagged β-Arrestin-2. Activation of HRH3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 24 Reagent, 500. (93-0563R24B)
AssayComplete Cell Plating 24 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress CXCR5 (BLR1) CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0514E2CP2L)
The PathHunter CXCR5 (BLR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL13/BCA-1/BLC) based activation of CXCR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR5 and EA-tagged β-Arrestin-2. Activation of CXCR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 14 Reagent, 500. (93-0563R14B)
AssayComplete Cell Plating 14 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress mCCR4 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0515E2MCP0L)
The PathHunter mCCR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL22/MDC) based activation of mCCR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR4 and EA-tagged β-Arrestin-2. Activation of mCCR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CALCRL-RAMP3 CHO-K1 β-Arrestin GPCR Assay, 2pl Kit. (93-0252E2CP0M)
The PathHunter CALCRL-RAMP3 (AM2) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. adrenomedullin) based activation of CALCRL GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCRL and EA-tagged β-Arrestin-2, along with untagged modulating protein RAMP3. Activation of CALCRL stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GRPR U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0229E3CP5M)
The PathHunter GRPR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. gastrin-releasing peptide) based activation of GRPR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GRPR and EA-tagged β-Arrestin-2. Activation of GRPR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress F2R (PAR1) CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0278E2CP0L)
The PathHunter F2R (PAR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. proteases, TFLLRN) based activation of F2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged F2R and EA-tagged β-Arrestin-2. Activation of F2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PPYR1 (NPY4R) CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0233E2CP0M)
The PathHunter PPYR1 (NPY4R) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. pancreatic polypeptide) based activation of PPYR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PPYR1 and EA-tagged β-Arrestin-2. Activation of PPYR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.